Professor of Biomedical Engineering
Dr. Wax's research interests include optical spectroscopy for early cancer detection, novel microscopy and interferometry techniques.
The study of intact, living cells with optical spectroscopy offers the opportunity to observe cellular structure, organization and dynamics in a way that is not possible with traditional methods. We have developed a set of novel spectroscopic techniques for measuring spatial, temporal and refractive structure on sub-hertz and sub-wavelength scales based on using low-coherence interferometry (LCI) to detect scattered light. We have applied these techniques in different types of cell biology experiments. In one experiment, LCI measurements of the angular pattern of backscattered light are used to determine non-invasively the structure of sub-cellular organelles in cell monolayers, and the components of epithelial tissue from freshly excised rat esophagus. This work has potential as a diagnostic method for early cancer detection. In another experiment, LCI phase measurements are used to examine volume changes of epithelial cells in a monolayer in response to environmental osmolarity changes. Although cell volume changes have been measured previously, this work demonstrates for the first time the volume of just a few cells (2 or 3) tracked continuously and in situ.
Appointments and Affiliations
- Professor of Biomedical Engineering
- Professor of Physics
- Member of the Duke Cancer Institute
- Faculty Network Member of the Duke Institute for Brain Sciences
- Bass Fellow
Contact Information
- Office Location: 2571 CIEMAS, Durham, NC 27708
- Email Address: a.wax@duke.edu
- Websites:
Education
- B.S. Rensselaer Polytechnic Institute, 1993
- M.A. Duke University, 1996
- Ph.D. Duke University, 1999
Research Interests
Optical spectroscopy for early cancer detection, novel microscopy and interferometry techniques.
Awards, Honors, and Distinctions
- Fellow. American Institute for Medical and Biological Engineering. 2014
- Fellow. International Society for Optics and Photonics. 2010
- Fellow. Optical Society of America. 2010
- Faculty Early Career Development (CAREER) Program. National Science Foundation. 2004
Courses Taught
- PHYSICS 621: Advanced Optics
- PHYSICS 493: Research Independent Study
- MOLCAN 551L: Biomedical Optical Spectroscopy and Tissue Optics (GE, IM)
- MENG 552: Master of Engineering Supplemental Internship
- MENG 551: Master of Engineering Internship/Project Assessment
- MENG 550: Master of Engineering Internship/Project
- GLHLTH 796T: Bass Connections Global Health Research Team
- GLHLTH 795T: Bass Connections Global Health Research Team
- GLHLTH 396T: Bass Connections Global Health Research Team
- GLHLTH 395T: Bass Connections Global Health Research Team
- EGR 491: Projects in Engineering
- EGR 393: Research Projects in Engineering
- ECE 541: Advanced Optics
- BME 791: Graduate Independent Study
- BME 552: Advanced Optics
- BME 551L: Biomedical Optical Spectroscopy and Tissue Optics (GE, IM)
- BME 550: Modern Microscopy (GE, IM)
- BME 494: Projects in Biomedical Engineering (GE)
- BME 493: Projects in Biomedical Engineering (GE)
- BME 436L: Biophotonic Instrumentation (DR)
In the News
- Follow the Ghostbusters: Crossing the Beams Brings Deeper Images Beneath the Sk…
- High-Speed Holography of Cells Spots Physical Beacons of Disease (Dec 1, 2021 |…
- Retinal Texture Could Provide Early Biomarker Of Alzheimer’s Disease (May 22, 2…
- Observing the Joint: Duke Engineers Adapt Imaging System to Make It Useful for …
- Low Cost & Clinically Accurate: New Retinal Scanner Could Prevent Blindness Wor…
- A Better Way to Measure the Stiffness of Cancer Cells (Mar 2, 2017 | Pratt Scho…
- Holographic Imaging and Deep Learning Diagnose Malaria (Sep 16, 2016)
Representative Publications
- Highland, R. E., A. Rancu, H. Price, S. M. Parker, M. Reynolds, B. D. Hoffman, and A. Wax. “Multimodal segmentation of dynamic subcellular features using quantitative phase imaging and FRET-based sensors [Invited].” Journal of the Optical Society of America A: Optics and Image Science, and Vision 41, no. 11 (November 1, 2024): C38–48. https://doi.org/10.1364/JOSAA.534440.
- Kendall, Wesley Y., Qinyi Tian, Shi Zhao, Seyedbabak Mirminachi, Erin O’Kane, Abel Joseph, Darin Dufault, et al. “Deep learning classification of ex vivo human colon tissues using spectroscopic optical coherence tomography.” J Biophotonics 17, no. 9 (September 2024): e202400082. https://doi.org/10.1002/jbio.202400082.
- Gupta, Deven K., Robert Highland, David A. Miller, and Adam Wax. “Utilizing quantitative phase microscopy to localize fluorescence in three dimensions via the transport of intensity equation.” Optics Letters 49, no. 15 (August 2024): 4457–60. https://doi.org/10.1364/ol.532991.
- Zhang, Haoran, Evan T. Jelly, David A. Miller, and Adam Wax. “Recovery of angular scattering profiles through a flexible multimode fiber.” Optics Express 32, no. 12 (June 2024): 21092–101. https://doi.org/10.1364/oe.522905.
- Jelly, Evan T., Zachary A. Steelman, Haoran Zhang, Kengyeh K. Chu, Cary C. Cotton, Swathi Eluri, Nicholas J. Shaheen, and Adam Wax. “Next-generation endoscopic probe for detection of esophageal dysplasia using combined OCT and angle-resolved low-coherence interferometry.” Biomedical Optics Express 15, no. 3 (March 2024): 1943–58. https://doi.org/10.1364/boe.515469.